ARS | Publication request: Identification of molecular markers associated with resistance to TSWV through genetic mapping

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 10, 2010
Publication Date: August 4, 2010
Citation: Qin, H., Li, Y., Guo, Y., He, G., Chen, C.Y., Culbreath, A., Knapp, S.J., Cook, D.R., Holbrook Jr, C.C., Wang, M.L., Guo, B. 2010. Identification of molecular markers associated with resistance to TSWV through genetic mapping [abstract]. Presented at the American Phytopathological Society Annual Meeting in Nashville, TN. on August 7-11, 2010.

Technical Abstract: Peanut is vulnerable to a range of diseases, such as tomato spotted wilt virus (TSWV), and early and late leaf spots. The objective of this study is to construct a genetic linkage map to facilitate quantitative trait locus (QTL) analysis and gene tagging for use in a marker-assisted breeding. Tifrunner has been released as a resistant cultivar to TSWV and leaf spots. New breeding line NC94022 has been identified with the highest resistance to TSWV. Two genetic mapping populations have been developed, a total of 248 F2:7s for Tifrunner x GT-C20 (T) and 352 F2:7s for SunOleic 97R x NC94022 (S). A total of 4574 simple sequence repeat (SSR) markers have been collected and screened among the parents of the populations for polymorphisms. Of the total SSR primer pairs, 269 and 173 primer pairs (markers) were polymorphic in these populations, respectively, and used in genotyping these RIL populations. Genotypes of the S population has been completed and the linkage map has been constructed which has 20 linkage groups (LG) with 186 mapped loci (173 SSRs and 13 with two loci). In 2009, we conducted field evaluation of F2:5s for disease resistance to TSWV with two replications, and one QTL for TSWV resistance have been identified with 2009 phenotype data. The phenotypic variation explained by this QTL was 40%. The seeds have been increased, multiple field phenotypes will be conducted in 2010, and the QTLs will be reevaluated. This map will be compared with the T population.