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United States Department of Agriculture

Agricultural Research Service

Research Project: BIOLOGY AND EPIDEMIOLOGY OF EMERGING PLANT PATHOGENIC OOMYCETES

Location: Foreign Disease-Weed Science

Title: Detection of Phytophthora ramorum chlamydospores in soil by baiting and dilution plating

Authors
item TOOLEY, PAUL
item Carras, Marie -

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: March 24, 2010
Publication Date: June 1, 2010
Citation: Tooley, P.W., Carras, M.M. 2010. Detection of Phytophthora ramorum chlamydospores in soil by baiting and dilution plating. Phytopathology. 100:S208.

Technical Abstract: Chlamydospores of P. ramorum produced by mixing 20 percent V8 juice broth cultures with sand and incubating over a 1 month period were used to infest field soil at concentrations ranging from 0.2 to 42 chlamydospores/cc soil. Chlamydospore recovery was determined by baiting with rhododendron leaf discs and dilution plating both when soil infestation was performed (time 0) and following 30 days storage at 4 C, as recommended in the soil and growing medium sampling protocol on the APHIS website (http://www.aphis.usda.gov/plant_health/plantpestinfo/pram /downloads/pdf_files/soil_growingprotocol.pdf). Baiting was slightly more sensitive than dilution plating at time 0, allowing detection of P. ramorum down to 0.2 chlamydospores/cc compared with 1 chlamydospore/cc for dilution plating. Following 30 days of infested soil storage at 4 C, P. ramorum was detected using both methods at significantly (P = 0.05) higher levels than at time 0. The results indicate that storage of P. ramorum-infested soil at 4 C for 30 days can enhance recovery of the pathogen.

Last Modified: 9/10/2014
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