Technical Abstract: Sources of post-natal infection with T. gondii include consumption of undercooked meat containing tissue cysts or ingestion of oocysts in contaminated food or environment. This study quantified the risk of acquiring infection from oocysts versus meat in pregnant women from the Valdivia Province, Chile by taking advantage of the detection of antibodies that specifically recognize a protein from the oocyst stage of the parasite. A total of 494 serum samples were available for testing along with some basic demographic information. Overall sero-prevalence was 39.1% and showed to be positively associated with age (p = 0.02) and negatively associated with college level education (p = 0.08). Among women with avidity index suggestive of infection within the last 6 months, 46.5% had evidence of oocyst-associated infection, which was particularly important in the very low socioeconomic group (p = 0.06). Public health interventions should target both sources of infection in this population.