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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND IMMUNE STRATEGIES TO CONTROL MUCOSAL PATHOGENS OF SWINE Title: Probing genetic control of swine responses to PRRSV infection: Current Progress of the PRRS Host Genetics Consortium.

Authors
item LUNNEY, JOAN
item Steibel, J -
item Reecy, J -
item Kerrigan, M -
item Trible, B -
item Rowland, B.B. -

Submitted to: International Symposium on Animal Genomics for Animal Health
Publication Type: Proceedings
Publication Acceptance Date: March 15, 2010
Publication Date: N/A

Technical Abstract: Understanding the role of host genetics in resistance to porcine reproductive and respiratory syndrome virus (PRRSV) infection, and the effects of PRRS on pig health and related growth, are goals of the PRRS Host Genetics Consortium (PHGC). The project uses a nursery pig model to assess pig resistance/susceptibility to primary PRRSV infection. To date, 6 groups of 200 crossbred pigs from high health farms were donated by commercial sources. After acclimation, the pigs were infected with PRRSV in a biosecure facility and followed for 42 days post infection (dpi). Blood samples were collected at 0, 4, 7, 10, 14, 21, 28, 35 and 42 dpi, and weekly weights recorded. All data have been entered into the PHGC relational database. Genomic DNA from all PHGC1-4 pigs were prepared and genotyped with the Porcine SNP60 SNPchip. Results have affirmed that all pigs become PRRSV infected with peak viremia from 4-21 dpi. Multivariate statistical analyses of viral load and weight data have identified PHGC pigs in different virus/weight categories. Sera are now being compared for factors involved in recovery from infection, including speed of response and levels of immune cytokines. These studies will compare data from PRRS resistant/maximal growth pigs to PRRS susceptible/reduced growth pigs. Overall, the PHGC project will enable researchers to verify important genotypes and phenotypes that predict resistance/susceptibility to PRRSV infection. The PHGC is funded by the US National Pork Board, USDA ARS, USDA NIFA PRRS CAP and functional genomic grants, NRSP8 Swine Genome and Bioinformatics Coordinators, and private companies.

Last Modified: 8/19/2014
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