Title: Using magnetic beads to reduce reanut allergens from peanut extracts. Authors
Submitted to: Journal of Allergy Clinical Immunology
Publication Type: Abstract Only
Publication Acceptance Date: March 4, 2010
Publication Date: March 5, 2010
Citation: Chung, S., Champagne, E.T. 2010. Using magnetic beads to reduce reanut allergens from peanut extracts. Journal of Allergy Clinical Immunology. 125(2)1:AB223. Technical Abstract: Ferric irons (Fe3+) and phenolic compounds have been shown to bind to peanut allergens. An easy way to isolate peanut allergens is by use of magnetic beads attached with or without phenolics to capture peanut allergens or allergen-Fe3+ complexes, thus, achieving the goal of producing peanut extracts with reduced levels of peanut allergens. Magnetic beads covalently attached with chlorogenic acid (CHL) (a phenolic) were prepared by rotating magnetic aminoethyl beads with a mixture of carbodiimide and chlorogenic acid. The resultant CHL-beads were then washed and treated with a regular peanut extract. Magnetic beads without CHL were treated with another peanut extract that had been incubated with Fe3+ and dialyzed. After beads treatment, both extracts were retrieved on a magnetic device that separated beads from the extracts. Protein/allergens that bound to the beads were retrieved by regenerating the beads with 1 M NaCl. The samples were then analyzed by SDS-PAGE and ELISA using a pooled plasma from peanut-allergic donors. Results showed that peanut extracts treated with magnetic CHL-beads exhibited a marked reduction in the levels of major peanut allergen Ara h 1 and a small reduction in Ara h 2. By contrast, both allergens were markedly reduced in the treated Fe3+-extracts. IgE bindings of both treated extracts were also reduced, as indicated by a 28-47% reduction in absorbance value. While further optimization is needed, the magnetic bead systems proved to be a simple way to partially remove peanut allergens from peanut extracts, and could be a potential approach to produce hypoallergenic peanut products and beverages.