|Bramwell, K -|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: March 1, 2010
Publication Date: March 16, 2010
Citation: Bramwell, K., Donoghue, A.M. 2010. Predicting Fertility. Determination of Holes Made by Sperm in the Perivitelline Layer of Laid Eggs: The Sperm Penetration Assay. Techniques for Semen Evaluation, Semen Storage and Fertility Determination [CD-ROM]. Version 2:90-94. St. Paul, MN: Midwest Poultry Federation. Technical Abstract: This procedure provides the quantitative determination of the rate of sperm penetration of the perivitelline layer (PL). This PL sperm penetration assay is performed with freshly laid eggs and can be used as a predictive measure of fertility. The original procedure, which was developed for chicken eggs (Bramwell et al., 1995), has been adapted to turkey eggs (Donoghue, 1996).