Author
DOGDOG, KHALED AL - Ain Shams University Of Cairo | |
RAGAB, M - Ain Shams University Of Cairo | |
FREEMAN, S - Volcani Center (ARO) | |
SPIEGEL, S - Volcani Center (ARO) | |
ATTIA, A - Agricultural Research Center Of Egypt | |
Martin, Robert |
Submitted to: International Horticultural Congress
Publication Type: Abstract Only Publication Acceptance Date: 3/28/2010 Publication Date: 8/23/2010 Citation: Dogdog, K., Ragab, M., Freeman, S., Spiegel, S., Attia, A., Martin, R.R. 2010. Molecular detection of some strawberry viruses in Egypt. International Horticultural Congress Abstract Book. Interpretive Summary: Technical Abstract: Strawberry plants exhibiting distinct virus-like symptoms (stunting, mottling, yellowing, vein clearing, vein necrosis and vein banding) were collected from strawberry production fields and nurseries in Qalubia Governorate, Egypt (about 20 km north of Cairo). Plants of 'Festival' and 'Sweet Charlie' were tested with Dot Blot ImmunoAssay (DBIA) using polyclonal antibodies specific for Strawberry latent ringspot virus (SLRSV) and Raspberry ringspot virus (RpRSV). In addition, total RNA was extracted from symptomatic strawberry plants and subjected to RT-PCR for further confirmation using primers specific for SLRSV, RpRSV, SMoV and SVBV (primer sequences from R.R. Martin). Amplicons obtained from the RT-PCR were 497 bp, 490 bp, 384 bp and 472 bp for RpRSV, SVBV, SLRSV and SMoV, respectively. The number of plants that indexed positive out of total tested for each virus including both methods was: SLRSV 19/28; RpRSV 36/58; SMoV 8/58; and SVBV 17/28. The SLRSV, SMoV and SVBV were transmitted by grafting to strawberry plants that subsequently developed curling, mottling, vein banding, vein necrosis and upward curling of the leaves and mechanically to Nicotiana tabacum 'White Burley' and to Chenopodium amaranticolor. After graft transmission each of the four viruses was detected in the grafted strawberry plants and SLRSV, SMoV and SVBV were detected in the plants inoculated mechanically. Based on the field inspection, positive serological and RT-PCR detection results, we concluded that these viruses infect strawberry and cause diseases in this crop in Egypt. The viruses were present in fruit production fields and in nurseries in the major strawberry production area in Egypt. |