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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #250159

Title: Raspberry latent virus, a New Reovirus Isolated from Crumbly Fruited Red Raspberry Plants

Author
item QUITO, D - Oregon State University
item Martin, Robert

Submitted to: International Horticultural Congress
Publication Type: Abstract Only
Publication Acceptance Date: 3/28/2010
Publication Date: 8/3/2010
Citation: Quito, D., Martin, R.R. 2010. Raspberry latent virus, a new reovirus Isolated from crumbly fruited red raspberry plants. International Horticultural Congress Abstract Book.

Interpretive Summary:

Technical Abstract: A virus induced crumbly fruit disease in 'Meeker' and other red raspberry cultivars has been observed in northern Washington, USA and British Columbia, Canada. Raspberry bushy dwarf virus (RBDV) and Raspberry leaf mottle virus (RLMV) were detected in raspberries with severe crumbly fruit. In addition, a novel reovirus, Raspberry latent virus (RpLV) that does not cause symptoms in single infections was isolated from these plants. Phylogenetic analyses demonstrate that RpLV is related to reoviruses belonging to the genera Oryzavirus, Cypovirus, Dinovernavirus and Fijivirus. The polymerase sequence has 35% aa sequence identity to Rice ragged stunt virus (RRSV, an oryzavirus). Reoviruses with an aa sequence identity greater than 30% in the RdRp sequence are generally considered members of the same genus (two exceptions have been reported). The analysis of the 5' and 3' terminal regions, indicate that RpLV and RRSV have different conserved sequence motifs, which suggests they are species from distinct genera. Furthermore, the first 3 nucleotides (AGU) at the 5' terminus of RpLV are conserved among members of the genera Cypovirus, Dinovernavirus, and Fijivirus, supporting the relatedness among these reoviruses. The lack of conservation between the terminal sequences of RpLV and RRSV, the very low aa identity in sequences from segments S8, S9 and S10, and the distinct hosts for these viruses justify the creation of a new genus for the classification of RpLV. These findings along with the lack of severe crumbly fruit symptoms in 'Meeker' red raspberry singly infected with RBDV in Oregon, suggest the existence of a novel virus complex associated with severe crumbly fruit in red raspberries. The complex may involve RBDV, RLMV and/or RpLV. Transmission studies are underway to determine the effect of each of these viruses singly and in all combinations on crumbly fruit symptom development in 'Meeker' red raspberry.