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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #250158

Title: Sequence Analysis of Raspberry latent virus Suggests a New Genus of Dicot Infecting Reoviruses

Author
item QUITO, D - Oregon State University
item Martin, Robert

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/24/2010
Publication Date: 6/3/2010
Citation: Quito, D., Martin, R.R. 2010. Sequence analysis of Raspberry latent virus suggests a new genus of dicot infecting reoviruses. Phytopathology. 100:S107.

Interpretive Summary:

Technical Abstract: Currently, there are three assigned genera of plant reoviruses: Phytoreovirus, Fijivirus and Oryzavirus. With only two exceptions, all plant reoviruses infect monocotyledonous plants. The recent characterization of Raspberry latent virus (RpLV) isolated from red raspberry plants in northern Washington has revealed new dicotyledonous hosts for plant reoviruses. Phylogenetic analyses demonstrate that RpLV is related to reoviruses belonging to the genera Oryzavirus, Cypovirus, Dinovernavirus and Fijivirus. Analysis of the polymerase sequence showed 35% aa identity to Rice ragged stunt virus (RRSV, an oryzavirus). As a general rule, reoviruses with an aa sequence identity greater than 30% in the RdRp sequence are considered members of the same genus (two exceptions have been reported). The analysis of the 5’ and 3’ terminal regions, however, indicate that RpLV and RRSV have different conserved sequence motifs, which would suggest they are species from distinct genera. Furthermore, the first 3 nucleotides (AGU) at the 5’ terminus of RpLV are conserved among members of the genera Cypovirus, Dinovernavirus, and Fijivirus, supporting the relatedness among these reoviruses. The lack of conservation between the terminal sequences of RpLV and RRSV, the very low aa identity in sequences from segments S8, S9 and S10, and the distinct hosts for these viruses justify the creation of a new genus for the classification of RpLV.