Technical Abstract: Legume seed-storage proteins, such as those from soybean and peanut, represent an important source of protein in the human diet. While generally well tolerated, these proteins also represent a potentially serious allergenic threat to many individuals. Despite high sequence similarity of several peanut allergens to their relatively hypoallergenic soy and kidney bean counterparts, it is not well understood why peanuts elicit such an acute allergenic response. We have employed a number of bioinformatics tools and strategies to further investigate the relationship between allergenicity and the primary/secondary/tertiary structure of legume seed-storage proteins. We find that mapping and comparison of multiple features (sequence conservation among classes, physical location of IgE epitopes, residues critical for IgE binding, etc.) on known and modeled structures provide for a better understanding of the relationship between legume protein structure, and the potential for novel legume varieties to elicit reduced allergenic response in the human population.