ARS | Publication request: Production of Truncated Candida antarctica Lipase B Gene Using Automated PCR Gene Assembly Protocol and Expression in Yeast for use in Ethanol and Biodiesel Production.

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 28, 2010
Publication Date: April 28, 2010
Citation: Hughes, S.R., Moser, B.R., Harmsen, A.J., Robinson, S., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.B., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2010. Production of truncated Candida antarctica Lipase B gene using automated PCR gene assembly protocol and expression in yeast for use in ethanol and biodiesel production [abstract]. Advanced Biofuels Leadership Conference. Talk 3. p. 5.

Technical Abstract: An improved column-based process for production of biodiesel was developed using a column containing a strongly basic anion-exchange resin in sequence with a column containing a resin to which a lipase biocatalyst is bound. Currently most biodiesel is produced by transesterification of triglycerides, such as corn oil, with short-chain alcohols, such as methanol or ethanol, with a strong base as a catalyst. The transesterification reaction to produce biodiesel can also be accomplished using resin-bound lipases as biocatalysts. In this study, two columns were prepared, one containing the strongly basic anion-exchange resin DIAION® and the other containing Candida antartica lipase B enzyme bound to Sepabead® EC-EP resin. These columns individually both catalyzed the transesterification of a mixture of ethanol and corn oil, producing comparable yields of ethyl esters (biodiesel). When run in sequence, it was demonstrated that the yield of ethyl esters increased by 20%.