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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR BIOLOGY AND GENOMICS OF FOODBORNE PATHOGENS Title: Top-down proteomic identification of protein biomarkers of food-borne pathogens using MALDI-TOF-TOF-MS/MS

Authors
item Fagerquist, Clifton
item Sultan, Omar

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: March 7, 2011
Publication Date: May 1, 2012
Citation: Fagerquist, C.K., Sultan, O. 2012. Top-down proteomic identification of protein biomarkers of food-borne pathogens using MALDI-TOF-TOF-MS/MS. Lee, M.S. Editor. Mass Spectrometry Handbook. New Jersey:John Wiley & Sons. p. 559-573.

Interpretive Summary: This chapter describes a step-by-step protocol and discussion of top-down proteomic identification of protein biomarkers of food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed in the Produce Safety & Microbiology Research Unit at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture.

Technical Abstract: This chapter describes a step-by-step protocol and discussion of top-down proteomic identification of protein biomarkers of food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed in the Produce Safety & Microbiology Research Unit at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture.

Last Modified: 11/27/2014
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