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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR AND GENETIC MECHANISMS OF FUNGAL DISEASE RESISTANCE IN GRAIN CROPS

Location: Crop Production and Pest Control Research

Title: The Draft Genome Sequence of Mycosphaerella fijiensis, the Black Sigatoka Pathogen of Banana

Authors
item Kema, Ghj -
item Goodwin, Stephen
item Van Der Lee, Taj -
item Dhillon, B -
item Arango, R -
item Crane, Charles
item Diaz, C -
item Souza, M -
item Carlier, J -
item Schmutz, J -
item Grigoriew, IV -

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: February 5, 2010
Publication Date: March 30, 2010
Citation: Kema, G., Goodwin, S.B., Van Der Lee, T., Dhillon, B., Arango, R., Crane, C.F., Diaz, C., Souza, M., Carlier, J., Schmutz, J., Grigoriew, I. 2010. The Draft Genome Sequence of Mycosphaerella fijiensis, the Black Sigatoka Pathogen of Banana. Meeting Proceedings.

Technical Abstract: Mycosphaerella fijiensis is a fungal pathogen of banana and the causal agent of the devastating Black Sigatoka or black leaf streak disease. Its control requires weekly fungicide applications when bananas are grown under disease-conducive conditions, which mostly represent precarious tropical environments. We started a multidisciplinary research program on M. fijiensis that is aiming at pesticide reduction. The first goal was to collect genomic data and to develop tools for molecular analysis of this pathosystem. Analyses of electrophoretic karyotypes on DNA extracted from protoplasts of M. fijiensis showed that chromosome sizes range between 500 kb and ~12 Mb. A genetic linkage map comprising 19 linkage groups covering 1417 cM was calculated using high LOD scores. All markers were sequenced and aligned to the draft 7.8x whole genome shotgun Sanger sequence of M. fijiensis CIRAD86. In addition more than 30,000 ESTs from three in vitro libraries were sequenced. The genome has an estimated size of 74 Mb and in 56 scaffolds covering more than 99% of the genome. The largest scaffold is 11.8 Mb in length and 28 scaffolds (99.8. %) are larger than 50 Kb. The genome size of M. fijiensis is 80% larger than that of M. graminicola mostly due to additional repeated sequences. The current draft release, version 1.0, includes a total of 10,327 gene models predicted and functionally annotated using the JGI annotation pipeline. The availability of the M. fijiensis genome will greatly assist future studies aimed at the control of black leaf streak disease as well as genomic comparisons with many other agronomically important Dothideomycetes fungi that currently are being sequenced through the Fungal Genome Program at the U.S. Department of Energy’s Joint Genome Institute.

Last Modified: 7/22/2014
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