Title: Antilisterial efficacy and lack of genotoxic potential of Listeria monocytogenes specific bacteriophages Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 1, 2010
Publication Date: March 7, 2010
Citation: Mackay, W., Sommers, C., Rajkowski, K.,Barton, D., 2010 Antilisterial efficacy and lack of genotoxic potential of Listeria monocytogenes specific bacteriophages [abstract].Annual Meeting of the Society of Toxicology. Salt Lake City, UT. p.1. Technical Abstract: Listeria monocytogenes, a psychrotrophic food-borne pathogen, is an occasional post-process contaminant on foods. In this study, the use of a commercial bacteriophage product was evaluated for the ability to inactivate L. monocytogenes inoculated (4-5 log CFU/cm2) onto raw catfish. Spray application of bacteriophage (7 log PFU/cm2) resulted in a 2 log reduction of L. monocytogenes on raw catfish stored for 6 days at 10 C. Published studies on the safety of bacteriophage products using in vitro genotoxicity assays are extremely limited. The bacteriophage preparation did not induce mutations in bacterial mutagenicity tests or 6-thioguanine resistant mutants in human TK6 lymphoblasts, either with or without exogenous metabolic activation. No induction of micronuclei (chromosome fragmentation) was observed in human TK6 lymphoblasts following exposure to bacteriophage, either with or without exogenous metabolic activation.