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United States Department of Agriculture

Agricultural Research Service

Research Project: AUGMENTATIVE BIOLOGICAL CONTROL AND MASS REARING FOR BENEFICIAL AND PEST INSECTS

Location: Biological Control of Pests Research Unit

Title: Expressed Sequence Reference Standards for Evaluating Stage-specific Gene Expression in Southern Green Lacewings, Chrysoperla rufilabris

Authors
item Allen, Margaret
item Walker Iii, William

Submitted to: Biocontrol Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 26, 2010
Publication Date: February 19, 2010
Repository URL: http://hdl.handle.net/10113/42889
Citation: Allen, M.L., Walker III, W.B. 2010. Expressed Sequence Reference Standards for Evaluating Stage-specific Gene Expression in Southern Green Lacewings, Chrysoperla rufilabris. Biocontrol Science and Technology. 20: 6, 589-593, doi: 10.1080/09853151003657969.

Interpretive Summary: The southern green lacewing, Chrysoperla rufilabris (Brumeister) is one of several generalist predators mass reared for sale to gardeners, horticulturists and vegetable producers in the United States. While these insects are quite amenable to mass rearing, they have not been studied as model organisms for genetic or many other research purposes. Thus, the availability of molecular tools for this organism is very limited. Molecular markers could be helpful in this insect, for studying its complex speciation, for estimating the overall health of mass-reared insects, and for identifying and understanding genes associated with insect predation. Five developmental stages, egg, young larva, mature larva, pupa, and adult, were tested using nine primer pairs. Three sequences were highly expressed at all life stages and six were differentially expressed. These primer pairs may be used as standards to quantitate functional gene expression associated with physiological processes such as reproduction, nutrition, and diapause.

Technical Abstract: Five developmental stages of Chrysoperla rufilabris were tested using nine primer pairs. Three sequences were highly expressed at all life stages and six were differentially expressed. These primer pairs may be used as standards to quantitate functional gene expression associated with physiological processes such as reproduction, nutrition, and diapause.

Last Modified: 8/22/2014
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