ARS | Publication request: Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust (Puccinia Striiformis f. sp. tritici) in a Hard Red Winter Wheat Germplasm IDO444

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 4, 2009
Publication Date: January 9, 2010
Citation: Chen, J., Souza, E.J., Guttieri, M.J., Chen, X., Chenggen, C., Xu, S., Hole, D., Zemetra, R. 2010. Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust (Puccinia Striiformis f. sp. tritici) in a Hard Red Winter Wheat Germplasm IDO444. Plant and Animal Genome Conference, Jan 9-13, 2010, Sand Diego, CA. Available at http://www.intl-pag.org/18/abstracts/P05c_PAGXVIII_303.html.

Interpretive Summary: Durable resistance to the wheat disease stripe rust is needed to prevent crop loss to this devastating disease. Durable resistance to stripe rust was identified in an Idaho winter wheat. Genetic markers place the resistance in two regions of the wheat genome and can be used to track the resistance in subsequent breeding efforts.

Technical Abstract: High-temperature adult-plant (HTAP) resistance to stripe rust (Puccinia striiformis f. sp. tritici) is a durable type of resistance in wheat. The objective of this study was to identify quantitative trait loci (QTL) conferring the HTAP resistance to stripe rust in a population consisted of 179 F7:8 recombinant inbred lines (RILs) derived from a cross between a susceptible cultivar ‘Rio Blanco’ and a resistant Idaho germplasm IDO444. The parents and RILs were evaluated for infection type and disease severity over two years in field trials under natural infection at Mt. Vernon and Pullman, WA. Genetic linkage maps were constructed based on data of 469 polymorphic molecular markers, including 108 SSRs and 361 DArTs. Two major QTL derived from IDO444 and one major QTL derived from Rio Blanco for HTAP resistance were consistently detected in all location-years. The three QTL were mapped on chromosomes 2B, 4A and 3BS, and explained 19 - 30%, 8 - 32%, and 8 – 20% of the total phenotypic variation, respectively. Of them, the 2B QTL is located in the region of the resistance QTL identified in cultivars ‘Louise’ and ‘Luke’, the 4A QTL is located in the region of the resistance QTL detected in a South Africa cultivar ‘Kariega’, while the 3BS QTL is located in the region of Yrns-B1 and the resistance QTL identified in a German line ‘Lgst. 79-47’. Molecular markers tightly linked to the three major QTL have potential to be used in the marker-assisted selection of HTAP resistance in wheat.