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United States Department of Agriculture

Agricultural Research Service

Research Project: EVOLUTIONARY ENZYMES AND SEPARATION PROCESSES FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES

Location: Bioproduct Chemistry and Engineering Research

Title: Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae

Authors
item Batt-Throne, Sarah
item Wan, David
item Doi, Roy -
item Wong, Dominic

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 18, 2009
Publication Date: March 1, 2010
Citation: Batt Throne, S.B., Wan, D., Doi, R.H., Wong, D. 2010. Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae. Meeting Abstract, 2010; CELL Poster Session; Poster #57 (Paper ID 18330).

Technical Abstract: Enzyme systems that digest the cellulose and hemicellulose in plant cell walls have potential value in the biorefining of feedstocks such as crop residues, straws, and grasses to fuel ethanol and other bioproducts. Clostridium cellulovorans is a useful source of biomass-degrading enzymes because this anaerobic bacterium produces cellulosomes – extracellular complexes of enzymes that degrade cellulose. The genes of several cellulosomal and non-cellulosomal enzymes were cloned into yeast expression vectors and transformed into the model eukaryotic organism Saccharomyces cerevisiae. Active endoglucanase, exoglucanase, and xylanase enzymes were constitutively expressed and secreted. These enzymes are being studied as prospective components of enzyme complexes for high-efficiency digestion of cellulosic and hemicellulosic biomass to fermentable sugars for biofuel production.

Last Modified: 7/24/2014
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