Location: Poultry Microbiological Safety Research
Title: Salmonella serotype diversity from broiler carcass rinses evaluated by two secondary enrichments along with two plating media Authors
Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: October 22, 2009
Publication Date: January 25, 2010
Citation: Cox Jr, N.A., Cray, P.J., Richardson, L.J., Buhr, R.J., House, S.L. 2010. Salmonella serotype diversity from broiler carcass rinses evaluated by two secondary enrichments along with two plating media. International Poultry Scientific Forum. January 25 - 26, 2010. Altanta, GA. Technical Abstract: Salmonella diversity on broiler carcasses has received significant attention in recent years, due to emphasis being placed on recovery of certain serotypes related to human attribution studies. The objective of this study was to evaluate Salmonella diversity from broiler carcass rinses by two cultivation methods. Twenty-six broiler carcasses were removed immediately after defeathering on two separate occasions and a whole carcass rinse procedure performed. The rinse was evaluated using a pre-enrichment (1% buffered peptone water) followed by transfer into either Gram negative (GN) or tetrathionate (TET) broth then from each plating onto BGS and XLT4 plates. If available, 3 typical colonies were picked per plate. A total of 236 isolates were identified as Salmonella and serotyping was performed by the National Veterinary Services Laboratories. Overall, eight different serotypes were recovered [S. Berta (n=48), S. Kiambu (n=35), S. Kentucky (n=79), S. Mbandaka (n=33), S. Heidleberg (n=14), S. Senftenburg (n=17), S. Agona (n=8), and S. Enteritidis (n=1)] with four of the serotypes accounting for 83% of the isolates (S. Kentucky, 33.5%, S. Berta, 20.3%, S. Kiambu, 14.8%, and S. Mbandaka, 14.4%). One group B isolate was classified as 4, 5, 12:1. In both visits to the plant, S. Kentucky and S. Berta were two of the prominent serotypes, however S. Kiambu was second most prevalent in visit 1, while S. Mbandaka was second most prevalent in visit 2. GN broth recovered significantly more S. Kentucky, while significantly (p=0.05) more S. Heldeberg and S. Senftenberg were isolated from TET. Significantly (p=0.05) more S. Kentucky was recovered from XLT4 than BGS originating from TET broth. S. Kiamba was detected 15 times on XLT4 where it went undetected on BGS. Salmonella diversity on broiler carcasses was evident by examination of the carcass rinse. The enrichment and plating media combinations along with number of colonies selected influenced the serotype recovered and demonstrates the bias that may occur during cultivation of Salmonella.