Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: INTERVENTIONS AND METHODOLOGIES TO REDUCE HUMAN FOOD-BORNE BACTERIAL PATHOGENS IN CHICKENS

Location: Poultry Microbiological Safety Research

Title: Pulsed Field Gel Electrophoresis along with Antimicrobial Resistance pattern of Salmonella serotypes isolated from broiler whole carcass rinses

Authors
item Cox, Nelson
item Cray, Paula
item Richardson, Larry
item Buhr, Richard
item McGlinchey, Beth
item Hall, Mary
item Haro, Jovita

Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: October 22, 2009
Publication Date: January 25, 2010
Citation: Cox Jr, N.A., Cray, P.J., Richardson, L.J., Buhr, R.J., Mcglinchey, B., Hall, M.C., Haro, J.H. 2010. Pulsed Field Gel Electrophoresis along with Antimicrobial Resistance pattern of Salmonella serotypes isolated from broiler whole carcass rinses. International Poultry Scientific Forum. January 25 - 26, 2010. Atlanta, GA.

Technical Abstract: Pulsed field gel electrophoresis (PFGE) and antibiogram patterns have been used to evaluate the diversity within and between individual Salmonella serotypes. The objectives of the study were to evaluate the PFGE along with antimicrobial resistance patterns of Salmonella isolates originating from broiler carcass rinses and compare pattern similarity to 4,620 XbaI patterns originating from testing 17,597 isolates in USDA-VetNet. The Salmonella isolates (n=173) originated from 52 post-pick carcass rinse samples using different cultivation method combinations. Overall, the antimicrobial resistance diversity as well as the PFGE diversity within eight different S. enterica serotypes [Agona (n=7); Berta (n=32); Heidelberg (n=8); I4, 5, 12:i:- (n=1); Kentucky (n=57); Kiambu (n=28); Mbandaka (n=27); Senftenberg (n=16)] was studied. For genetic fingerprinting, PFGE was used and to determine antibiogram patterns, isolate resistance to 15 different antibiotics was evaluated using a SensititreĀ®. Overall, 8 different antibiogram patterns was observed across all isolates. S. Kentucky isolates (n=57) exhibited the greatest heterogenecity with 6 different antimicrobial resistance patterns within 13 different PFGE patterns. S. Berta (n=32) isolates exhibited 3 different PFGE patterns while S. Agona exhibited 2 different patterns. Isolates among both serotypes were pan-susceptible. Compared to patterns within the USDA-VetNet database, a total of 10 new Salmonella PFGE XbaI patterns were identified with 6 new patterns originating from S. Kentucky isolates. The S. Kiambu isolates all had a unique XbaI pattern (TEN01.0013ARS) that had only been observed in two previous isolates from diagnostic cattle. The identification of new patterns suggests that further work needs to be conducted on cultivation influences not only Salmonella serotype diversity but diversity within serotypes. The isolates from this study originated from cultivation method combinations that are not commonly used by regulatory agencies to recover Salmonella from poultry carcasses.

Last Modified: 7/22/2014