Submitted to: Molecular Ecology Resources
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 22, 2010
Publication Date: May 23, 2010
Citation: Rinehart, T.A., Trigiano, R., Wadl, P., Johnson, D., Pooler, M.R., Scheffler, B.E., Hadziabdic, D. 2010. Characterization of twelve microsatellite markers for the native redbud tree (Cercis canadensis). Molecular Ecology Resources. 10(4):751-754.
Interpretive Summary: Eastern redbud is widespread in forests along the eastern U.S. Trees typically grow in bottomlands and along forest edges and are adaptable to diverse environments. Eastern redbud is also a popular ornamental tree or shrub with profuse blooms in early spring. Native populations are easily located in the wild during peak bloom times and serve as an important genetic resource, both for native plant conservation and as genetic material for ornamental plant breeders seeking to improve eastern redbud cultivars. Here we developed molecular markers to better understand the population biology of native stands and for horticultural improvement of cultivated forms.
Eight microsatellite DNA markers were developed for studies of gene flow in the redbud tree (Cercis canadensis), which is native to North America. The loci were unlinked and polymorphic in a sample of 22 individuals collected from a single population in Oak Ridge, Tennessee. Allele number ranged from 2 to 11 per locus and observed heterozygosity ranged from 0.091 to 0.850. All loci were in Hardy-Weinberg equilibrium.