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United States Department of Agriculture

Agricultural Research Service

Research Project: RESPONSE OF DIVERSE RICE GERMPLASM TO BIOTIC AND ABIOTIC STRESSES

Location: Dale Bumpers National Rice Research Center

Title: Structural and functional analysis of rice blast avirulence gene AVR-Pita1 in Magnaporthe oryzae

Authors
item Dai, Yuntao -
item Jia, Yulin
item Correll James, -
item Lee, Fleet -

Submitted to: Experiment Station Bulletins
Publication Type: Experiment Station
Publication Acceptance Date: August 12, 2009
Publication Date: N/A

Technical Abstract: The resistance gene Pi-ta in rice has been effectively deployed around the globe to prevent the rice blast disease. It was previously known that Pi-ta interacts with the avirulence gene AVR-Pita1 in the fungus in triggering effective resistance responses. In order to predict the stability of deployed Pi-ta in commercial rice cultivars a population of 250 field fungal isolates were collected from major rice production areas worldwide to study the structural and functional properties of AVR-Pita1. Nucleotide variation at AVR-Pita1 was observed in avirulent isolates toward Pi-ta. Structure changes including frame-shift and partial and complete deletions were identified to be new mechanisms for “defeating” the Pi-ta resistance gene. The AVR-Pita1 gene was shown to restore Pi-ta-mediated resistance once it was introduced into three virulent US isolates. These findings suggest that the structural alteration of AVR-Pita1 may impact its roles in resistance and pathogenicity.

Last Modified: 9/10/2014
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