ARS | Publication request: Molecular Characterization of two Potato Virus S Isolates from Late Blight Resistant Genotypes of Potato (Solanum tuberosum)

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 28, 2009
Publication Date: October 10, 2009
Repository URL: http://riley.nal.usda.gov/nal_web/digi/submission.html
Citation: Y-H., Druffel K.L., Whitworth J., Pavek M.J., Pappu H.R. 2009. Molecular characterization of two Potato Virus S isolates from late blight resistant genotypes of potato (Solanum tuberosum). Archives of Virology. 154:1861-1863

Interpretive Summary: Potato virus S (PVS) is transmitted by aphids and mechanical transmission. Symptoms in potato range from latent (no symptoms) to deepening of the leaf veins, roughness of the leaf surface, mosaic patterns on the leaves and early drop of leaves. Yield loss is reported. Three PVS isolates collected from late blight resistant potato cultivars and a clone were compared at the molecular level with other PVS isolates. Two of the isolates were subjected to a complete genomic sequence and were shown to be most closely related to each other and an isolate from Syria and all clustered with the PVS Ordinary group.

Technical Abstract: Potato virus S (PVS) has a widespread distribution in the U.S. However, only two complete nucleotide sequences have been published. A recent survey of potato fields in the state of Washington confirms that PVS is widely prevalent. Late blight resistant (LBR) potato cultivars and genotypes were shown to be highly susceptible to PVS. Isolates were collected and characterized at the molecular level including the complete genomic sequence of two isolates from the LBR potato genotypes in this study. A phylogenetic tree was constructed and showed that three isolates from the U.S. were most closely related to an isolate from Syria and clustered in the PVS Ordinary group.