ARS | Publication request: Molecular characterization and assessment of genetic diversity in valencia mini core using SSR markers

Submitted to: American Peanut Research and Education Society Proceedings
Publication Type: Proceedings
Publication Acceptance Date: July 14, 2009
Publication Date: February 1, 2010
Citation: Kottapalli, P., Upadhyaya, H., Kottapalli, K.R., Payton, P.R., Puppala, N. 2010. Molecular characterization and assessment of genetic diversity in valencia mini core using SSR markers. In: 2009 Proceedings of The American Peanut Research and Education Society, Inc. July 14-17, 2009, Raleigh, North Carolina. 41:38.

Technical Abstract: Valencia peanuts known for their premium taste are mainly grown in eastern New Mexico and west Texas of the US. A well characterized and structured germplasm is a prerequisite for any crop improvement program. Recently a Valencia core was developed from the USDA collection using 26 morphological descriptors. In this study we attempted an extensive characterization of genetic diversity and relationships in the core subset using microsatellite or simple sequence repeat (SSR) markers. One hundred and twelve genotypes from the Valencia core were genotyped with 36 SSR markers generating 500 polymorphic loci. A moderate level of genetic variation was observed among the core subset with genetic distances ranging from 0.1 to 0.54. The available variation in the Valencia germplasm can be utilized for selection of diverse parents for breeding and development of mapping populations.