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United States Department of Agriculture

Agricultural Research Service

Research Project: ECONOMIC COMPETITIVENESS OF RENEWABLE FUELS DERIVED FROM GRAINS AND RELATED BIOMASS

Location: Sustainable Biofuels and Co-Products

Title: Lipid analysis via HPLC with a charged aerosol detector

Author
item Moreau, Robert

Submitted to: Lipid Technology
Publication Type: Review Article
Publication Acceptance Date: October 8, 2009
Publication Date: November 2, 2009
Citation: Moreau, R.A. 2009. Lipid analysis via HPLC with a charged aerosol detector. Lipid Technology. 21:191-193.

Technical Abstract: Most lipid extracts are a mixture of saturated and unsaturated molecules. Therefore, the most successful HPLC detectors for the quantitative analysis of lipids have involved the use of “universal” or “mass” detectors such as flame ionization detectors (FID) and evaporative light scattering detectors (ELSD). Recently a new type of HPLC “universal” detector, a charged aerosol detector (CAD), was developed and is now commercially available. This detection method involves nebulizing the HPLC column effluent, evaporating the solvents, charging the aerosol particles and measuring the current from the charged aerosol flux. During the approximately four years that the charged aerosol detector has been commercially available, several publications have described HPLC-CAD methods for lipid analysis. The major results of these publications will be summarized in this report.

Last Modified: 10/1/2014
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