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United States Department of Agriculture

Agricultural Research Service

Research Project: METABOLIC VARIABLES AFFECTING THE EFFICACY, SAFETY, AND FATE OF AGRICULTURAL CHEMICALS Title: Analytical Method for the Determination of Chlorate in Animal Matrices

item Smith, David
item Taylor, Joshua
item Bahnson, Peter -

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 12, 2009
Publication Date: August 16, 2009
Citation: Smith, D.J., Taylor, J.B., Bahnson, P. 2009. Analytical Method for the Determination of Chlorate in Animal Matrices. In: Program of American Chemical Society National Meeting and Exposition, August 16-20, 2009, Washington, D.C. Program Vol. 77, Abstract #42, page 96-97.

Technical Abstract: Contamination of meat products with gram-negative enteric pathogens contribute to over 1.5 million food borne illnesses each year. Recent studies have demonstrated 2-3 log unit reductions in numbers of E. coli O157:H7 and(or) Salmonella in food animals dosed with an experimental chlorate-containing product. Sensitive and specific analytical methods for chlorate in food animal tissues or in gastrointestinal fluids do not exist, but would be useful. To this end, an analytical method specific for chlorate in animal matrices was developed. The method involves fortification of samples with 18O-chlorate internal standard, extraction of chlorate in water, solid-phase extraction cleanup of the aqueous phase, dilution with water, and analysis by LC-MS-MS using either triple-quadrapole or quadrapole-time-of-flight detection. Across 3 analysts, recoveries of chlorate in fortified swine feces and sheep serum (51 to 2500 ng/g) ranged from 99 to 110% and 95 to 104%, respectively. Blank matrices showed no significant interferences. Analyses of gastrointestinal tissues from hogs dosed with chlorate salts were highly repeatable.

Last Modified: 11/26/2015
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