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United States Department of Agriculture

Agricultural Research Service

Research Project: MICROBIAL CATALYSTS TO PRODUCE FUEL ETHANOL AND VALUE ADDED PRODUCTS Title: Production of the Anaerobic GMAX-L Yeast Using High-Throughput Mating and Transformation of Saccharomyces cerevisiae With Identified Genes For Simultaneous Cellulosic Ethanol and Biodiesel Production

Authors
item Hughes, Stephen
item Rich, Joseph
item Moser, Bryan
item Doll, Kenneth
item Bischoff, Kenneth
item Qureshi, Nasib
item Jones, Marge -
item Tasaki, Ken -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 10, 2009
Publication Date: September 10, 2009
Citation: Hughes, S.R., Rich, J.O., Moser, B.R., Doll, K.M., Bischoff, K.M., Qureshi, N., Jones, M., Tasaki, K. 2009. Production of the anaerobic GMAX-L yeast using high-throughput mating and transformation of Saccharomyces cerevisiae with identified genes For simultaneous cellulosic ethanol and biodiesel production [abstract]. Bradley Biology Chemisty Seminar Series.

Technical Abstract: Tailored GMAX-L yeast engineering for strains capable of universal ethanol production industrially with coproduction of an expressed lipase catalyst for coproduction of ethyl esters from corn oil and ethanol from the modern dry grind ethanol facility: Production of the stable baseline glucose, mannose, arabinose, xylose-utilizing (GMAX) yeast will be evaluated by taking the genes identified in high-throughput screening for a plasmid-based yeast to utilize xylose and glucose anaerobically for ethanol production. The use of the xylose isomerase gene from Piromyces in combination with a modified xylulokinase gene from bacteria plus the anaerobic growth genes from S. cerevisiae allowed anaerobic growth on xylose and glucose simultaneously for cellulosic ethanol production and to be the background strain for other co-product gene expressions such as a lipase or an insecticidal genes. The resulting stable transformed plasmids into any industrial yeast strains of Saccharomyces cervisiae that are already tolerant to environments in the production biorefinery are being developed for universal ethanol production from any feedstock provided. Initially a cellulosic strain will be produced with XI, XKS, and one or more of the anerobic xylose utilization genes for use on acid or base hydrolysates for high level production of cellulosic ethanol. This strain will be capable of universal sugar utilization for ethanol production and express the lipase catalyst for resin based ethyl ester biodiesel production.

Last Modified: 8/29/2014
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