ARS | Publication request: Discrete Variation Of Glycitein Ratio In Soybean (Glycine max) Hypocotyls

Submitted to: Soybean Research World Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: June 5, 2009
Publication Date: August 9, 2009
Citation: Berger, M., Artigot, M., Nelson, R.L., Dayde, J. 2009. Discrete Variation Of Glycitein Ratio In Soybean (Glycine max) Hypocotyls [abstract]. [abstract]. In: Proceedings of the World Soybean Research Conference VIII,August 9-16, 2009, Beijing, China. 2009 CDROM.

Technical Abstract: Soybean seed has a very uneven isoflavone concentration and composition, with 4 to 10 times more isoflavones in the hypocotyls than in the cotyledons, and a low genistein ratio in the hypocotyls. Independent accumulation kinetics of isoflavones with distinct controls are observed in these two seed parts. This suggests that it may be possible to breed soybean for desired isoflavone compartmentalization in the seed. Depending on the cultivar, hypocotyls have either a high (55%) or low (30%) glycitein ratio and it is influenced very little by the environment. A germplasm accession with no glycitein was identified within the USDA Soybean Germplasm Collection. Near isogenic lines with either high or low glycitein ratios were selected from within the cultivar Dwight. Hypocotyls from 276 F2 seeds obtained from reciprocal crosses between cultivars having respectively high and low glycitein ratio were individually analyzed. The results were compared with the hypocotyl glycitein concentration in seeds of 74 pairs of F4 sister plants produced from the same cross. These results were consistent with a single gene inheritance with codominance and no maternal effects. A cross has been made between the glycitein null line and a standard cultivar. Seeds from 60 F2 and 60 F3 plants were analyzed. Results also indicated single gene control and codominance. The isolines were studied in two locations during two years. Isoflavone concentrations displayed year and location effects but the lines were stable for their hypocotyl glycitein types. Variation observed in these experiments can be useful in breeding for isoflavone concentration and providing material to investigate glycitein synthesis.