|Kelley, Rowena -|
Submitted to: In Vitro Biology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 8, 2009
Publication Date: June 6, 2009
Citation: Sakhanokho, H.F., Kelley, R.Y. 2009. Induced Tetraploidy in Hedychium bousigonianum Via Treatment of Embryogenic Callus with Antimitotic Agents. Proc.Soc. In vitro Biol. Conf . p. S70. Technical Abstract: The genus Hedychium belongs to the Gingiberaceae family and includes both diploid and tetraploid species. H. bousigonianum is a diploid (2n = 34) species with fragrant flowers, a desired and targeted trait in Hedychium breeding. The objective of this study was to induce tetraploidy in this species so that it can be hybridized with tetraploid Hedychium species or cultivars to develop improved cultivars. A protocol for this purpose was developed by treating embryogenic callus with various concentrations of colchicine (2.5, 5, and 10 mM) or oryzalin (30, 60, 120 µM) for 24, 48, and 72 hours. Ploidy level of regenerated plants was determined using flow cytometry, stomatal frequency and length, chloroplast number, and chromosome counts. Triploid, tetraploid, and mixoploid regenerated plants were obtained with both antimitotic agents. The most efficient treatments for inducing tetraploidy were the 2.5 mM colchicine for 24, 48, or 72 hours and the 60 µM oryzalin for 72 hours.