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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR MICROBIOLOGY AND CONTROL OF ENTERIC PATHOGENS THAT CONTAMINATE FRESH PRODUCE Title: Tandem Repeat Stability in Escherichia coli O157:H7 is Dependent on Environmental Stress

item Cooley, Michael
item Carychao, Diana
item Nguyen, Kimberly
item Whitehand, Linda
item Mandrell, Robert

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 10, 2010
Publication Date: N/A

Technical Abstract: Multilocus variable number tandem repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication, but changed after irradiation, elevated temperatures and starvation conditions. Plasmid, pO157, was lost frequently under these stresses. Environmental stresses may increase phylogenetic diversity measured by MLVA.

Last Modified: 8/27/2015
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