Technical Abstract: Map-based cloning and manipulation of genes controlling important traits for crop remains a great challenge due to the complex of crop genomes and lack of a high resolution of genetic and physical maps. In this review article, we compared the various mapping methods available for plant research and introduce radiation hybrid mapping technology potential useful for generating high resolution physical maps for complex genomes such as wheat. Unlike genetic mapping, radiation hybrid mapping is not recombination- based; making marker distribution more evenly along a chromosome. Therefore, the use of a combination of genetic and physical mapping methods can be very useful for advancing knowledge on the number and distance between genes of interests as well as to better understand the topography and organization of the genome.