METABOLIC VARIABLES AFFECTING THE EFFICACY, SAFETY, AND FATE OF AGRICULTURAL CHEMICALS
Location: Animal Metabolism-Agricultural Chemicals Research
Title: Estrogenic Activity and Estrogens of Surface Waters in Proximity to a Large Dairy
Submitted to: Society of Environmental Toxicology and Chemistry Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 31, 2009
Publication Date: November 20, 2009
Citation: Shappell, N.W., Billey, L.O., Elder, K.H. 2009. Estrogenic Activity and Estrogens of Surface Waters in Proximity to a Large Dairy. Society of Environmental Toxicology and Chemistry (SETAC)Abstract Book, New Orleans, LA, November 19-23, 2009. Abstract 31, page 11.
Large concentrated animal farms have been the subject of intense public scrutiny, in part due to concern about environmental release of endocrine disruptors. To assess the potential impact of a large dairy herd (greater than 2000 milking head) using best management practices, area surface waters and drain tile run-off were evaluated for estrogenic activity (E-Screen) and estrogens (LC MSMS). Samples were collected before and after application of wastewater (as lagoon liquor, solids, and press effluent) via center pivot irrigation and soil injection. To assess background E-activity, samples were collected upstream from the municipal wastewater treatment plant (WWTP), downstream of WWTP, below animal housing, below sites of field application, and from two drain tile sites. Total N and P were - evaluated, in addition to water volume and flow parameters. Fifty four thousand tons of waste (from ~1,000 milking head) were applied to ~2,000 acres. The post-application E2Eq values were similar to preapplication values, with the exception of drain tile samples which increased by ' 2, and all surface waters had E2Eq activity below the no observable effect concentration for E2 of 1ng/L. Manure slurries were extracted by liquid solvents, followed by three separate SPE columns. In spite of rigorous clean-up, matrix effects resulted in ion suppression that precluded confidence in quantitation of estrogens. With this caveat, relative concentrations were: estrone > 17a-E2 > 17ß-E2. These results indicate field application of animal wastes using best management practices do not have to increase the biological estrogenic activity of surrounding surface waters.