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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPING BIOCONVERSION PROCESSES FOR HIGH-VALUE CARBOHYDRATE PRODUCTS

Location: Renewable Product Technology Research Unit

Title: Sugars, Stable Isotopes, and Spectrometry: New Methods for the Analysis of Carbohydrate Metabolism

Authors
item Price, Neil
item Meiss, Ernst -
item Fischer, Markus -
item Vermillion, Karl

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 4, 2009
Publication Date: December 4, 2009
Citation: Price, N.P., Vermillion, K. 2009. Sugars, Stable Isotopes, and Spectrometry: New Methods for the Analysis of Carbohydrate Metabolism [abstract]. International Symposium on Glycoconjugates. Program/Abstract 127. 26:186.

Technical Abstract: Structural analysis of carbohydrates involves three parameters: composition, linkage, and conformation, and tends to rely on the various forms of two techniques; mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. These techniques are enhanced and extended by the use of stable isotopes, especially **13Carbon and deuterium. Because these isotopes can be selectively detected they are typically used in metabolic pulse-chase experiments similar to radiolabels. This presentation will focus on three newer aspects: 1. GC-MS analysis of global metabolic labeling from 13C precursor sugars; 2. Hydrogen-deuterium exchange mass spectrometry (HX-MS) for oligosaccharide conformational studies; and 3. Stable isotope-enhance diffusion-ordered **13C-NMR spectroscopy (SIE-DOSY **13C-NMR). Applications and advantages of these techniques for the study of microbial carbohydrate metabolism will be discussed.

Last Modified: 8/29/2014