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United States Department of Agriculture

Agricultural Research Service

Research Project: Pacific Fruit Genetic Resource Management and Sustainable Production Systems

Location: Tropical Plant Genetic Resources and Disease Research

Title: Protection and conservation of Caricaceae germplasm with PRSV resistant transgenic papaya

Authors
item MATSUMOTO BROWER, TRACIE
item Zee, Francis
item Suzuki, Jon -
item Tripathi, Savarni -
item MACKEY, BRUCE
item Hollingsworth, Robert
item Shintaku, Michael -
item KEITH, LISA

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 8, 2009
Publication Date: July 18, 2009
Citation: Matsumoto, T.K., F.T.P. Zee, J.Y. Suzuki, S. Tripathi, B. Mackey, R. Hollingsworth, M. Shintaku and L. Keith. 2009. Protection and conservation of Caricaceae germplasm with PRSV resistant transgenic papaya. Plant Biology 2009. Honolulu, HI. P09021 pg. 166.

Technical Abstract: Papaya ringspot virus (PRSV) is a devastating disease that has a detrimental impact on both commercial papaya production and Caricaceae germplasm conservation. The PRSV coat protein transgenic line 55-1 and derived progeny are resistant to PRSV and have saved the papaya industry in Hawaii. Here we present preliminary information on a method to protect susceptible Caricaceae germplasm using PRSV resistant transgenic papayas as border planting to limit aphid transmitted infection. Similar to transgenic crops throughout the world there is public concern on cross contamination of transgenic material into non-transgenic lines. As the designated germplasm repository for Caricaceae we are responsible for maintaining the genetic integrity of each accession. Therefore, we have also developed a protocol utilizing polymerase chain reaction (PCR) for the detection of adventitious presence of transgenic material in both the parental plants and the resulting seed population by testing for the 55-1 transformation event to assure a 99.9% chance of obtaining greater than 99.5% transgene free seeds. The protocol developed in this study is not typical for most seed validation techniques since there is a higher than normal rejection rate for rejecting seed lots; however, we believe this is necessary to ensure the genetic integrity of seeds stored in the repository.

Last Modified: 8/19/2014