|Campbell, Michael -|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 30, 2009
Publication Date: July 1, 2009
Repository URL: http://abstracts.aspb.org/pb2009/public/P21/P21002.html
Citation: Campbell, M.A., Suttle, J.C. 2009. Potato Tubers Treated with Sprout Inhibitors Exhibit Transcript Profiles That Have Similarities to the Dormant State [abstract.] Plant Biology 2009 Abstracts. P21002. Available: http://abstracts.aspb.org/pb2009/public/P21/P21002.html. Technical Abstract: The compounds chlorpropham (CIPC) and 1,4-dimethylnaphthalene (DMN) are used to suppress sprout growth and prolong the storage of potato tubers. However, the mechanism of action for these chemicals is poorly defined. Microarray analysis and quantitative real-time PCR were utilized to compare the transcript profiles of dormant, nondormant, and potato tubers treated with CIPC, and DMN. Natural progression from the dormant to the nondormant state demonstrated a decrease in ABA inducible transcripts particularly in the BURP class of proteins. Exposure to CIPC and DMN resulted in the expression of similar class of ABA inducible transcripts suggesting that sprout inhibitors may function via a prolongation of dormancy induced growth suppression. CIPC treatment resulted in decreased expression of transcripts associated with plastid development and metabolism. DMN resulted in an increase in expression of transcripts encoding for proteins involved with osmotic regulation, specifically osmotin and germin-like proteins. Levels for transcripts encoding for genes involved with cell division such as PCNA, KIP1, and KIP2, were measured using quantitative PCR. PCNA expression was linked to rapidly dividing meristems, while KIP1 and KIP2 expression was associated with dormant meristems or meristems treated with CIPC or DMN.