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Title: 454 pyrosequencing project on expressed genes from the New World Screwworm, Cochliomyia hominivorax

Author
item Guerrero, Felicito
item Saldivar, Leonel
item DOWD, S - Research And Testing Laboratories, Llc
item DJIKENG, A - J Craig Venter Institute
item WILEY, G - University Of Oklahoma
item MACMIL, S - University Of Oklahoma
item NAJAR, F - University Of Oklahoma
item ROE, B - University Of Oklahoma

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 9/9/2008
Publication Date: 9/9/2008
Citation: Guerrero, F., Saldivar, L., Dowd, S.E., Djikeng, A., Wiley, G., Macmil, S., Najar, F., Roe, B.A. 2008. 454 pyrosequencing project on expressed genes from the New World Screwworm, Cochliomyia hominivorax. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nlm.nih.gov. Assession number SRA001820.5

Interpretive Summary: The New World screwworm, Cochliomyia hominivorax, was a devastating pest of livestock and other animals, including humans, throughout the US, Mexico and Central America. The female fly deposits its eggs at a wound site and the larval stage feeds on living tissue. During periods of heavy fly infestation and oviposition, death of the affected animal will often follow without human intervention. In the 1950s, annual losses to the US livestock industry topped $120 million. Although eradicated from North America, the screwworm still is a pest in South America and the Caribbean. Reinfestation of North America is prevented by a sterile male release program conducted in Panama. We synthesized four DNA libraries from expressed genes of screwworm, using egg, second instar larvae, adult male, and adult female as source material, and obtained 46,695, 64,686, 62,853, and 49,650 sequences from the libraries, respectively. The 4 sets of sequences have been submitted and published under GenBank Accession Number SRA001820.5.

Technical Abstract: The New World screwworm, Cochliomyia hominivorax, was a devastating pest of livestock and other animals, including humans, throughout the US, Mexico and Central America. Although eradicated from North America, the screwworm still is a pest in South America and the Caribbean. Reinfestation of North America is prevented by a sterile male release program conducted in Panama. We synthesized four cDNA libraries from expressed genes of screwworm, using egg, second instar larvae, adult male, and adult female as source material, and obtained 46,695, 64,686, 62,853, and 49,650 sequences from the libraries, respectively.The 4 sets of sequences have been submitted and published under GenBank Accession Number SRA001820.5.