Technical Abstract: Tillage and fertilization practices used in row crop production are thought to alter greenhouse gas emissions from soil. Thus, a field experiment was conducted at the Sand Mountain Research and Extension Center located in the Appalachian Plateau region of Northeast Alabama on a Hartsells fine sandy loam. Measurements of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) emissions were evaluated using GRACEnet protocols to assess the effects of different tillage (conventional vs. no-tillage) and fertilizer application (subsurface banding vs. surface application) practices in a corn cropping system. In addition, stover weight, ear dry weight, grain yield, nitrogen (N) content of stover and grain, root dry weight, and root pull force were quantified to understand how these management practices impacted crop response. Fertilizer sources consisted of urea-ammonium nitrate (UAN), ammonium nitrate (AN) and poultry litter (M) applied at a rate of 170 kg ha-1 of available N. Surface application of fertilizers resulted in the greatest concentration of gaseous loss compared to banding of fertilizers. Conventional tillage practices also resulted in higher concentrations of gas emissions compared to no-tillage practices. These results suggest that organic fertilizers and banding practices increased greenhouse gas emissions, while inorganic fertilizers and banding practices resulted in higher yields.