Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2009
Publication Date: July 14, 2009
Citation: Guo, B. Holbrook Jr., C.C. Chen C.Y. 2009. Development of peanut genetic "Road Map" for marker-assisted breeding. Proceedings of the American Peanut Research and Education Society Annual Meeting. July 14-17, 2009. Raleigh, N.C. Technical Abstract: In the southeastern U.S., tomato spotted wilt virus disease has become a major limiting factor for many peanut producers, while the control methods are limited. Both early (Cercospora arachidicola) and late (Cercosporidium personatum) leaf spot diseases are among the worst foliar diseases of cultivated peanut. Leaf spot disease control in the U.S. has depended mainly on routine applications of the fungicides, either on a calendar or advisory schedule. Infection of peanut with Aspergillus parasiticus and consequent contamination with aflatoxin, a by-product of fungal metabolism and the most potent naturally-occurring carcinogen, are a serious threat to regional agricultural production and to human and animal safety. The objectives of this research are to develop a genetic linkage map and to conduct QTL (quantitative trait loci) studies of interest traits, such as resistance to TSWV, leaf spots, and reduction in aflatoxin contamination. A genetic map constructed from a population segregating for a trait of interest is required for QTL identification and marker development. Two RIL (recombinant inbred line) mapping populations have been constructed from crosses of Tiftrunner x GT-C20 and SunOleic 97R x NC94022. The populations were advanced to the F4 by single seed descent. Individual plants were harvested and progeny rows were grown to produce the F4:6 RIL populations. The populations consisted of 248 individual lines for Tifrunner x GT-C20 and 356 individual lines for SunOleic 97R x NC94022. In 2009, we will phenotype and genotype these populations. These populations will be made available for the community and the collaborators for marker development and QTL studies.