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United States Department of Agriculture

Agricultural Research Service

Research Project: COMBINING CONVENTIONAL AND MOLECULAR TECHNIQUES TO IMPROVE SEED COTTON VALUE

Location: Crop Genetics Research Unit

Title: Identification and Genomic Location of a Reniform Nematode (Rotylenchulus reniformis) Resistance locus (Ren_ari) Introgressed from Gossypium aridum into Upland Cotton (G. hirsutum)

Authors
item Romano, Gabriela
item Sacks, Erik
item Stetina, Salliana
item Robinson, Arin
item Fang, David
item Gutierrez, Osman -
item Scheffler, Jodi

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 28, 2009
Publication Date: December 1, 2009
Citation: Romano, G.B., Sacks, E.J., Stetina, S.R., Robinson, A.F., Fang, D.D., Gutierrez, O., Scheffler, J.A. 2009. Identification and Genomic Location of a Reniform Nematode (Rotylenchulus reniformis) Resistance locus (Ren_ari) Introgressed from Gossypium aridum into Upland Cotton (G. hirsutum). Theoretical and Applied Genetics. 120:139-150

Interpretive Summary: In recent years economic loses due the reniform nematode have been increasing; this parasite feeds on cotton plant roots and consequently decreases the production of fiber. Although chemical control and crop rotation can help to control this cotton pest, ultimately, the best choice, from an environmental and human health perspective as well as for economic reasons, is to have cotton plants that are resistant to reniform nematode. Because there is no resistance to reniform nematode in upland cotton, but resistance exists in other species, genetic material from those species was introduced into upland cotton. In this study, we identified the location of newly introduced resistance into the upland cotton genome, and we developed molecular tools (DNA markers) to tag this resistance. These tools will aid breeders and geneticists in the quest to produce cotton cultivars with a high level of resistance to reniform nematode by providing a quick way to assess if new plant selections possess resistance.

Technical Abstract: In this association mapping study, a tri-species hybrid, [G. arboreum × (G. hirsutum × G aridum)], was crossed with MD51ne (G. hirsutum) and progeny from the cross were used to identify and map SSR markers associated with reniform nematode (Rotylenchulus reniformis) resistance. Seventy six progeny (the 50 most resistant and 26 most susceptible) plants were genotyped with 104 markers. Twenty-five markers were associated with a resistance locus which we designated Ren_ari and two markers, BNL 3279_132 and BNL 2662_090, mapped within 1 cM of Ren_ari. Because the SSR fragments associated with resistance were found in G. aridum and the bridging line G 371, G. aridum is the likely source of this resistance. The resistance is simply inherited, possibly controlled by a single dominant gene. The markers identified in this project are a valuable resource to breeders and geneticists in the quest to produce cotton cultivars with a high level of resistance to reniform nematode.

Last Modified: 7/30/2014
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