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United States Department of Agriculture

Agricultural Research Service

Research Project: FUNCTIONAL GENOMICS FOR IMPROVING NUTRIENTS AND QUALITY IN ALFALFA AND SOYBEAN

Location: Plant Science Research

Title: Transcription Profiling and Mutation Detection of Soybean Homoeologous Genes

Authors
item Stupar, Robert -
item Lin, Jer-Young -
item Jackson, Scott -
item Bolon, Yung Tsi
item Muehlbauer, Gary -
item Naeve, Seth -
item Orf, James -
item Vance, Carroll
item Haun, William -

Submitted to: Soybean Research World Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: June 18, 2009
Publication Date: July 13, 2009
Citation: Stupar, R.M., Lin, J., Jackson, S.A., Bolon, Y.E., Muehlbauer, G.J., Naeve, S., Orf, J.H., Vance, C.P., Haun, W.J. 2009. Transcription profiling and mutation detection of soybean homoeologous genes [abstract]. In: Developing a Global Soy Blueprint for a Safe Secure and Sustainable Supply, World Soybean Conference Research VIII, August 9-16, 2009, Beijing, China. p. 109. Available: www.wsrc2009.cn/en/index.asp.

Technical Abstract: The soybean genome maintains numerous gene duplications, many of which are derived from ancient large-scale duplication. We are interested in exploring the evolutionary fate of duplicated genes and the extent to which gene duplication affects selectable trait variation. We are applying quantitative SNP-based methods to monitor the transcriptional and genetic variation of duplicated genes in soybean. Transcriptional assays based on SNPs were designed for 29 homoeologous gene pairs residing on soybean linkage groups A2 and E (chromosomes 8 and 15, respectively). We determined transcriptional level variations among the gene pairs for a range of different soybean tissue types and genotypes. Thus, we were able to explore the effects of intraspecific and tissue-specific variation on homoeologous gene expression. Additionally, we are interested in examining the effect of extensive homoeologous gene deletion on plant phenotype. We are currently using the LGA2-LGE quantitative SNP assays to identify heterozygous and homozygous fast neutron-induced deletions in the homoeologous regions.

Last Modified: 10/1/2014
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