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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR STRATEGIES FOR DETECTION AND IDENTIFICATION OF NEMATODES INFECTING FORAGE LEGUMES AND DEVELOPMENT OF HOST RESISTANCE Title: Molecular and morphological description of Meloidogyne arenaria from traveler’s tree (Ravenala madagascariensis)

item Skantar, Andrea
item Carta, Lynn
item Handoo, Zafar

Submitted to: Society of Nematology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 13, 2009
Publication Date: N/A

Technical Abstract: An unusual variant of Meloidogyne arenaria was discovered on roots of a traveler’s tree (Ravenala madagascariensis) intended for display at a public arboretum in Pennsylvania. The population aroused curiosity by the lack of visible galling on the roots of the infected plant. Morphometrics of the population fit within the ranges reported for M. arenaria, with a mosaic of features in common with either M. platani or other tropical Meloidogyne spp. Molecular characterization included analysis of four loci. The mitochondrial sequence, extending from cytochrome oxidase II (COII) to the 16S (1RNA) gene, was nearly identical to another M. arenaria population and closely related to sequences from M. morocciensis and M. thailandica. The 28S D2-D3 expansion segment was most similar to those from M. arenaria, M. incognita and M. paranaensis, and the IGS-2 was most related to those from M. thailandica, M. arenaria and M. incognita. Analysis of partial Hsp90 genomic sequences revealed the greatest similarity to M. arenaria, M. thailandica and an Hsp90 haplotype from M. floridensis, and a composite sequence comprised of EST from M. arenaria. While highly unusual in some aspects, there were no molecular or morphological features that clearly set this population apart as a new species; taken together, the data points to its identification as M. arenaria.

Last Modified: 7/27/2016