ARS | Publication request: Influence of cultural methodology on Salmonella serovar detection and serogroup recovery from broiler carcass rinses

Submitted to: Poultry Science Association
Publication Type: Abstract Only
Publication Acceptance Date: March 20, 2009
Publication Date: July 20, 2009
Citation: Cox Jr, N.A., Richardson, L.J., Cray, P.J., Ladely, S.R., Buhr, R.J. 2009. Influence of cultural methodology on Salmonella serovar detection and serogroup recovery from broiler carcass rinses. Poultry Science Association.88(S1):431P, P.132.

Technical Abstract: Consumption of or contamination from raw or undercooked poultry products containing Salmonella serovars can cause acute bacterial gastroenteritis in humans. Salmonella serovars recovered from human patients and poultry carcasses have been compared to determine the relationship of poultry to human illness. The objectives of this study were to evaluate the sensitivity of four methodology procedures for Salmonella recovery from broiler carcass rinsates and influence of these procedures on the diversity of Salmonella serogroups recovered. Two replications were performed, each with carcasses (n=26) procured directly from a commercial processing plant after defeathering. Carcasses were individually bagged and transported on ice to the laboratory. Each carcass was rinsed with 100 mL of buffered peptone for 1 min and the rinsate collected. Aliquots (1 mL) of rinsate were then inoculated into GN Hajna (GN) and Tetrathionate (TET) broth. Both broths were incubated at 37°C and at 24h a 0.1 mL of GN broth was transferred to Rappaport-Vassiliadis (RV) media. At 48h, 0.1 mL of the TET broth was transferred to RV. RV tubes were incubated at 37°C for 24h then streaked for isolation onto two different selective agar plates, BG Sulfa (BGS) and XLT-4. Following 24h incubation at 37°C, presumptive colonies (a maximum of 3/plate) were selected and standard conformational procedures performed. Of the 52 carcass rinsates, Salmonella was recovered from 41, 46, 34, and 45 by the media combinations of GN-BGS, GN-XLT-4, TET-BGS, and TET-XLT-4, respectively. GN detected Salmonella in 46/52 of the carcass rinsates and 45/52 by TET. Salmonella was recovered from 49/52 carcass rinsates using XLT-4 plating media and 47/52 by BGS plating media. Five serogroups of Salmonella were detected by all methods (B, C1, C3, D1, and E). GN broth in combination with either plating media selected for a significantly (P>0.05) greater number of group C3 Salmonella but significantly (P>0.05) fewer group E Salmonella. These data suggest that the enrichment and plating media used for Salmonella detection from chicken carcass rinsates can influence the sensitivity of recovery as well as the serogroup recovered.