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United States Department of Agriculture

Agricultural Research Service

Title: Cloning biomass-degrading enzymes from environmental sources

Authors
item Lee, Charles
item Wong, Dominic
item Wagschal, Kurt
item Orts, William

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 13, 2009
Publication Date: February 26, 2009
Citation: Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract.

Technical Abstract: Our laboratory is focused on discovering and engineering new enzymes that hydrolyze lignocellulosic biomass. In this presentation, I will discuss the metagenomic DNA approaches that we use. We collect samples of microorganisms from the environment and isolate the DNA from the entire population. This material is used to create genomic DNA libraries. We screen these libraries for desired activities. Activity assays and expression constructs that were specifically designed for high throughput screening will be described.

Last Modified: 12/20/2014