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Research Project:
APPLICATION OF BIOLOGICAL AND MOLECULAR TECHNIQUES TO THE DIAGNOSIS AND CONTROL OF AVIAN INFLUENZA AND OTHER EMERGING POULTRY PATHOGENS
Location: Exotic and Emerging Avian Viral Diseases Research Unit
Title: Simultaneous detection and identification of influenza virus types, subtypes and emergent variants using re-sequencing microarrays
Authors
 | Tibbetts, C - TESSARAE, LLC | | Borsuk, L - TESSARAE, LLC | | Lichanska, A - TESSARAE, LLC | | Lorence, M - TESSARAE, LLC | | Weslowski, B - TESSARAE, LLC | | Schafer, K - TESSARAE, LLC | | Stenger, D - NAVAL RESEARCH LAB | | Lin, B - NAVAL RESEARCH LAB | | Malanowski, A - NAVAL RESEARCH LAB | | Wang, Z - NAVAL RESEARCH LAB | | Blaney, K - NAVAL RESEARCH LAB | | Long, N - NAVAL RESEARCH LAB | | Schnur, J - NAVAL RESEARCH LAB | | Metzgar, D - NAVAL HEALTH RESEARCH CT | | Myers, C - NAVAL HEALTH RESEARCH CT | | Faix, D - NAVAL HEALTH RESEARCH CT | | Russell, K - NAVAL HEALTH RESEARCH CT | | Brown, J - NAVAL HEALTH RESEARCH CT | | Saad, M - NAVAL MED RU-3, EGYPT | | Thomas, Colleen | |
Swayne, David
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Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 4, 2010
Publication Date: February 3, 2010
Citation: Tibbetts, C., Borsuk, L., Lichanska, A., Lorence, M., Weslowski, B., Schafer, K., Stenger, D., Lin, B., Malanowski, A., Wang, Z., Blaney, K., Long, N., Schnur, J., Metzgar, D., Myers, C., Faix, D., Russell, K., Brown, J., Saad, M., Thomas, C., Swayne, D.E. 2010. Simultaneous detection and identification of influenza virus types, subtypes and emergent variants using re-sequencing microarrays. PLOS One 5(2):e8995, doi:10.1371/journal.pone.0008995.
Interpretive Summary: Rapid and accurate detection, identification and genetic characterization are essential for effective monitoring for influenza viruses. This report describes applications of a new biotechnology method capable of simultaneous obtaining gene sequences of influenza virus and 29 other viral and bacterial pathogens that have been associated with respiratory illness. The new assay was better at detecting and subtyping influenza viruses, than other molecular methodologies. This will save time and reduce the cost for identifying respiratory pathogens.
Technical Abstract:
Rapid and accurate detection, identification and genetic characterization are essential for effective surveillance and epidemiological tracking of influenza viruses. This report describes applications of a resequencing pathogen microarray (RPM) assay that is capable of simultaneous sequencing of subtypes of Type A and Type B influenza virus hemagglutinin and neuraminidase genes; selected influenza virus matrix (M), NS and PB2 genome segments; and multiple representative genes of 29 other viral and bacterial pathogens that have been associated with influenza-like illness. The RPM assay has equivalent to greater sensitivity and superior specificity for detection and subtype identification of influenza viruses, and it delivers significantly greater decision-quality information per assay than traditional serological subtyping assays or more recently adopted polymerase chain reaction (PCR)-based methodologies.
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