ARS | Publication request: Expression Profiling Using New Generation Sequencing Technologies

Submitted to: Developmental and Comparative Immunology
Publication Type: Proceedings
Publication Acceptance Date: March 16, 2009
Publication Date: August 30, 2009
Citation: Waldbieser, G.C. 2009. Expression Profiling Using New Generation Sequencing Technologies. Developmental and Comparative Immunology. P: 305

Interpretive Summary: Microarray hybridization technology has become widely used in parallel analysis of gene expression. Recent advances in genome sequencing platforms point to an alternate approach through digital quantitation of sequencing reads produced from cDNA samples. This presentation will compare advantages and limitations of transcript profiling using existing and emerging high-throughput sequencing platforms versus microarray hybridization approaches.

Technical Abstract: Microarray hybridization technology has become widely used in parallel analysis of gene expression. Recent advances in genome sequencing platforms point to an alternate approach through digital quantitation of sequencing reads produced from cDNA samples. This presentation will compare advantages and limitations of transcript profiling using existing and emerging high-throughput sequencing platforms versus microarray hybridization approaches.