ARS | Publication request: Genomic Studies in Soybean: Toward Understanding Seed Oil and Protein Production

Submitted to: International Conference on Legume Genomics and Genetics
Publication Type: Abstract Only
Publication Acceptance Date: December 7, 2008
Publication Date: December 7, 2008
Citation: Bolon, Y.E., Joseph, B., Cannon, S.B., Diers, B., Farmer, A., Graham, M.A., May, G., Muehlbauer, G., Specht, J., Tu, Z., Weeks, N., Xu, W., Shoemaker, R.C., Vance, C.P. 2008. Genomic Studies in Soybean: Toward Understanding Seed Oil and Protein Production [abstract]. IV International Conference on Legume Genomics and Genetics, December 7-12, 2008, Puerto Vallarta, Mexico. Abstract No. W28. p. 101 and Abstract No. P57, p. 63.

Technical Abstract: The molecular mechanisms that influence soybean seed composition are not well understood. Insight into the genetic controls involved in these traits is important for future soybean improvement. In this study, we identified candidate genes at the major soybean protein quantitative trait locus at Linkage Group I using near-isogenic lines that differ in seed protein and oil. Genome-wide transcript profiles were obtained from these lines using GeneChip (R) and high-throughput sequencing technologies. Variations between the profiles presumably reflect changes related differences in seed protein and oil. These results demonstrate the power of transcriptome analyses to contrast near-isogenic lines and illustrate how gene profiling technologies may aid in the annotation of genes in the soybean genome. Further investigation may provide new insight into the genes and pathways involved in protein and oil accumulation in the soybean seed.