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United States Department of Agriculture

Agricultural Research Service

Title: Genetic Determinants of Responses to Selenium Supplementation

Authors
item COMBS, GERALD
item ZENG, HUAWEI
item Jackson, Matthew
item Johnson, Luann - UNIV OF NORTH DAKOTA
item Hoeg, Antonia - NATL CANCER INSTITUTE
item Schomburg, Lutz - INST OF EXP ENDOCRIN
item Davis, Cindy - NATL CANCER INSTITUTE
item Milner, John - NATL CANCER INSTITUTE

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: January 7, 2009
Publication Date: April 27, 2009
Repository URL: http://www.fasebj.org
Citation: Combs, G.F., Zeng, H., Jackson, M.I., Johnson, L.K., Hoeg, A., Schomburg, L., Davis, C.D., Milner, J.A. 2009. Genetic Determinants of Responses to Selenium Supplementation. Journal of Federation of American Societies for Experimental Biology. 23:346.3.

Technical Abstract: In a cohort of healthy adults (106 M, 155 W) in eastern North Dakota, we determined the relationships of five biomarkers of selenium (Se) status (plasma Se, serum selenoprotein P [SePP], plasma glutathione peroxidase [GPX3] activity, buccal cell Se, urine Se) to genotype for four selenoproteins (cytosolic glutathione peroxidase [GPX1], phospholipid hydroperoxide glutathione peroxidase [GPX4], the 15 kD selenoprotein [SeP15], SePP) and two glutathione S-transferases (GST-M1, GST-T1). The cohort had plasma Se of 141.5±23.7 (SD) ng/ml, SePP lof 3.55 (CI 2.61, 4.51) mg/L, GPX3 activities of 3.64±0.54 nmoles NADPH/min/mg protein, buccal cell Se of 10.2±6.5 ng Se/mg protein, and urinary Se of 58.2±21.5 ng Se/mg creatinine. Plasma Se was significantly related to GPX3 genotype (198Leu/Leu: 135.7a±19.0 ng/ml; 198Leu/Pro: 139.2a,b±23.8 ng/ml; 198 Pro/Pro: 145.9b±24.4 ng/ml; P<0.05). Buccal cell Se was significantly related to SeP15 genotype (C/C: 8.77a [5.85, 13.14]; T/C: 7.65b [5.06, 11.57]; T/T: 8.27a,b [6.16, 11.10] P<0.05). SePP was significantly related to SePP genotype at a polymorphism in the 3’-UTR region (A/A: 3.49a,b [2.91, 4.18]; G/A: 3.24 a [2.44, 4.31]; G/G: 3.62 b [2.75, 4.76]), but not one in the coding region (A234T). No other effects were detected. These results show that genetic polymorphisms can significantly affect some biomarkers used to assess Se status.

Last Modified: 7/28/2014
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