Technical Abstract: Moisture is a critical parameter that influences many aspects of cotton fiber from harvesting and ginning to various fiber properties. Because of their importance, reference moisture methods that are more accurate than the existing oven-drying techniques and relatively easy to generate results are necessary. One such method is the Karl Fischer Titration technique, which is based on the chemical reaction between water, iodine and sulfur dioxide in a non-aqueous medium. The moisture in a sample is titrated with iodine. Used in 126 ASTM reference methods, the Karl Fischer approach is popular because of its precision, its small sample size and simple sample preparation, as well as its ability to measure water with a high degree of specificity. In this paper, cotton fiber moisture is measured in 13 cottons by fully automated KFT instrumentation that uses a novel oven design to extract moisture in solid samples, a dry sweep gas to transport the released moisture to the titration cell, and software to detect the end point. Analysis time is about 5 min per specimen. The mean measured moisture levels, compared to two traditional oven-drying methods, were 0.43 % lower with a standard deviation about half that of the oven procedures.