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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR TOOLS TO MINIMIZE RISK IN GENETICALLY ENGINEERED CROPS

Location: Crop Improvement & Utilization Research

Title: Intragenic Applications for Wheat Genetic Engineering

Author
item BLECHL, ANN

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2009
Publication Date: January 15, 2009
Citation: Blechl, A.E. 2009. Intragenic Applications for Wheat Genetic Engineering (abstract). Crop Improvement and Utilization Intragenics Workshop. p. 6.

Technical Abstract: Biolistics is the most common method used for wheat transformation. In a typical experiment, separate plasmids containing a gene of interest and a selectable marker gene are co-bombarded into embryogenic callus. The two genes are usually co-integrated into a single site in the host genome. The use of "clean genes", plant expression cassettes that have been stripped of their plasmid backbones, facilitates recovery of wheat plants containing only the gene of interest without any linked marker genes. This presentation will outline the intragenic approach for wheat biolistic transformation and present two examples of applications expected to improve bread-making quality.

Last Modified: 9/10/2014
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