RESEARCH, ACQUISITION, MANAGEMENT, AND DOCUMENTATION OF PLANT GENETIC RESOURCES
Location: Plant Germplasm Introduction and Testing
Title: Genetic diversity analysis of 122 Lupin PI lines using AFLP markers.
| Iqbal, M - ISSR-IALR VIRGINIA |
| Bhardwaj, H - VIRGINIA STATE UNIV |
| Ahsan, R - ISRR-IALR VIRGINIA |
Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 1, 2008
Publication Date: October 16, 2008
Citation: Iqbal, M., Coyne, C.J., Bhardwaj, H., Ahsan, R. 2008. Genetic diversity analysis of 122 Lupin PI lines using AFLP markers. Agronomy Abstracts. Annual Meeting ASA-CSSA-SSSA October 4-9, Houston, TX. p 658-9.
Interpretive Summary: Lupin is an important legume around the world. In the early part of 20th century, it has been used as a cover crop to improve soil nitrogen in several parts of the country. However, due to the phenomenal success of soybean in US agriculture system and the presence of some undesirable alkaloids in older varieties, it did not receive due attention as a major legume crop. USDA, by a concerted effort, has collected a large number of PI lines for breeding and cultivation in US from various parts of world. The objective of this study was to analyze the genetic diversity of the collected germplasm. We used fluorescent labeled AFLP markers for exploring the genetic diversity of lupin PI lines in the USDA germplasm collection. DNA was isolated using DNAeasy plant mini kit (QIAgen, CA) according to the manufacturer’s instructions. AFLP analysis was carried out according to the Vos et al. (1995) with some modifications. AFLP fragments were analyzed using AFLP 600 size standard (Beckman Coulter, Fullerton, CA) using Fragment analysis software and scored as 1 and 0 for present and absence.
A total of 20 EcoR1+Mse1 primer combinations were used for the AFLP analysis of the 122 Lupin PI lines. However, 2 did not produce consistent amplification profiles among majority of the varieties and therefore were dropped from the analysis. The 18 primer pairs amplified a total of 2277 fragments that were detected by the CEQ 8800 genetic analysis system. The data set was analyzed with NTSYSpc Numerical Taxonomy and Multivariate Analysis System version 2.2 and genetic similarities were determined using various coefficients of similarities. The results will help in understanding the genetic distances or similarities/dis-similarities of the collected germplasm. The information about the genetic relationships among the PI lines will help breeders make decisions on selecting parents to develop new and improved lupin cultivars.