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United States Department of Agriculture

Agricultural Research Service

Research Project: INTEGRATED MANAGEMENT OF PESTS AFFECTING COTTON: PLANT GENETICS, BIOCONTROL, AND NOVEL METHODS OF PEST ESTIMATION Title: DNA sequences from two SSRs (CIR316 and MUCS088) linked to root-knot nematode resistance genes from diverse cottons (Gossypium spp).

Authors
item Ulloa, Mauricio
item Roberts, Philip - UNIV. OF CA-RIVERSIDE

Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: December 31, 2008
Publication Date: May 5, 2010
Citation: Ulloa, M., Roberts, P.A. 2010. DNA sequences from two SSRs (CIR316 and MUCS088) linked to root-knot nematode resistance genes from diverse cottons (Gossypium spp).. Genbank. GeneBank accessions: FJ599673-FJ599688.

Technical Abstract: We investigated DNA sequencing information from alleles (DNA amplified fragments) of two previously reported SSR markers (CIR316 and MUCS088) linked to root-knot nematode (RKN) resistance genes. Markers based on electrophoretic differences, including RFLPs, AFLPs and SSRs can sometimes mask underlying genetic variation, complicating marker discovery and application in the complex polyploid cotton genome. Single-nucleotide polymorphism (SNP) allows scientists to access sequence differences directly by single-base variations in the genetic code usually represented as two, or sometimes three, different bases at a single position. We selected three major Acala/Upland germplasm sources of RKN resistance [NemX (N901), Clevewilt 6, and Auburn 634]. In addition, we selected four RKN susceptible sources (PS 6, PS 7, 3-79, TM1), one moderately resistant source (WMJJ), two A (A1 and A2), and three D (D1, D5, and D8) diploid genome species for discovering DNA differences and genome similarities. After multiple alignments and verification of DNA sequences from all entries, 15 DNA sequences were selected from SSR MUCS088, and 11 DNA sequences were selected from SSR CIR316. The 15 selected MUCS088 DNA sequences varied in size from 141 bp to 153 bp, and the 11 CIR316 varied from 189 bp to 207 bp. These sequences will be deposited in GenBank (NCBI: http://www.ncbi.nlm.nih.gov). Discovered DNA sequence differences and similarities from these entries will be useful for examining the introgression of RKN resistance and its ancestral genome origin.

Last Modified: 8/21/2014
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